The complete chloroplast genome of Agrimonia pilosa Ledeb. isolated in Korea is 155,125 bp long (GC ratio is 36.9\%) and has four subregions: 84,458 bp of large single copy (34.9\%) and 18,737 bp of small single copy (30.4\%) regions are separated by 25,965 bp of inverted repeat (42.6\%) regions including 129 genes (84 protein-coding genes, eight rRNAs, and 37 tRNAs). 258 SNPs and 542 INDELs were iden- tified as intraspecific variations against the partial genome (KY419942). Phylogenetic trees show that our chloroplast genome was clustered with the previous A. pilosa chloroplast genome.