Dysphania pumilio (R.Br.) Mosyakin & Clemants which belongs to Amaranthaceae is an invasive species all over the world. In this study, we presented first complete chloroplast genome of D. pumilio of which length is 151,962 bp consisting four sub regions: 83,758 bp of large single copy (LSC) and 17,742 bp of small single copy (SSC) regions are separated by 25,231 bp of inverted repeat (IR) regions. One hundred and twenty-eight genes (84 coding genes, eight rRNAs, and 36 tRNAs) were annotated successfully. The overall GC contents of the chloroplast genome were 36.9\% and in the LSC, SSC and IR regions were 34.8\%, 30.4\%, and 42.7\%, respectively. First chloroplast genome of Dysphania will provide accurate phylogenetic position of Dysphania genus among neighbour genera.