R&D CENTER

Whole genome sequence of new species of Plantago as a second whole genome in Plantaginaceae

Jongsun Park, Chan-Ho Park, Yongsung Kim
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Plantago sp. is a recently identified species in Korea, which shows expended leaf-like bract unlike small bract of Plantago depressa, which is neighbor species based on molecular phylogenic analysis. To dissect second whole genome of Plantaginaceae, we sequenced this species with four different libraries of Illumina sequencer. In total, 102Gbp were generated, estimated as 117x coverage. We successfully generated 722 Mb genomes, which is slightly smaller than k-mer analysis results (869Mb). 218,230 ORFs were predicted by AUGUSTUS among which 33.22\% (72,497) contain known functional domains detected by InterPro Scan. 40,697 simple sequence repeats (SSRs) were identified from plantago genome, while Mimulus guttatus, belonging to the same order, Lamiales, contains 84,751 SSRs. 404 cytochrome P450s involved in various biochemical pathways of plant secondary metabolites were found, which is larger than that of M. guttatus genome. This genome is accessible in the Plantago Genome Database (http://www.plantagogenome.info/) which equipped with several bioinformatics tools for comparative genomics researches.