R&D CENTER

Enhancements of the Integrated Platform for Invasive Pests (IPIP) with the pipeline of COI-BLAST

Jongsun Park1,2,*, Donghyun Kim1,2, Hong Xi1,2, Jonghyun Park1,2, Wonhoon Lee3, Kwan-Seok Lee4
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Accumulated researches for invasive insect pests have presented that various pests have been already settled down or are in progress to invasion to Korea: e.g., Solenopsis invicta, Linepithema humile, Metcalfa pruinosa, and Anoplolepis gracilipes. The Integrated Platform for Invasive Pests (IPIP; http://ipip.infoboss.co.kr:2020/) which was developed for managing molecular sequences of invasive insect species have been improved: 1) Organelle genome browsers based on GenomeArchive® was implemented to collect complete mitochondrial genomes of invasive and exotic pests, 2) The pipeline to classify collected COI and COI sequences based on their absolute positions was developed by utilizing BLAST search against representative organelle genome sequences, and 3) COI-BLAST, newly developed web-based tool, was established to display BLAST results together with COI/COII positions as well as phylogentic tree constructed with homologous COI/COII sequences. Based on these newly implemented functions of the IPIP, we expected that our platform can be utilized not only to understand their origin or haplotype of invasive and exotic pests but also to identify them quickly via obtaining marker sequences from the collected mixed samples. Moreover, several automated pipelines in the IPIP will help us maintain this system efficiently.