R&D CENTER

Genome Survey of Two Korean Liverworts, Marchantia polymorpha and Dumortiera hirsuta

Woochan Kwon, Yongsung Kim, Juhyeon Min, and Jongsun Park*
URL  
Liverwort is a basal group of land plants. Recently, one of model organism of liverwort, Marchantia polymorpha, was sequenced and published, presenting that total length is 228 Mb with special sex chromosomes. For understanding liverwort genomes in detail, we sequenced M. polymorpha and Dumortiera hirstua isolated in Korea. M. polymorpha belongs to the family Marchantiaceae, which is a basal family in order Marchantiales and D. hirstua which is the only species in family Dumortieraceae is a second basal group. 11.2 Gbp raw reads of M. polymorphia (K0749) were generated by Illumina HiSeq 4000 and only 59.71\% raw reads were aligned against M. polymorpha genome covering 175.02 Mb and identifying 490,932 SNPs and 65,364 INDELs (2.43 variations per 1 kb). Based on de novo assembly of K0749, 18,729 SSRs were identified, which is higher than those of the reference genome (16,129 SSRs), presenting that genome structure of K0749 is quite different from the reference genome. 20.1 Gb raw reads of D. hirstua were sequenced by HiSeq 4000 and estimated genome size is 2.803 Gbp by k-mer analysis. Current de novo assembled sequences presented 1.08 Gbp with N50 is 700bp. We will generate more sequence of D. hirstua to finalize genome sequences, from which we can understand evolutionary history of basal group of family Dumortieraceae evolutionary history at genome-wide scale.